Journal: Cell Reports Medicine

Article Title: Engineered human myogenic cells in hydrogels generate innervated vascularized myofibers within dystrophic mouse muscle on long-term engraftment

doi: 10.1016/j.xcrm.2025.102019

Figure Lengend Snippet: Long-term engraftment of human myofibers and human PAX7+ cells in the satellite cell niche with innervation and vascularization in mdx nude mice (A) A representative transverse cryosection of mdx nude mouse TA muscle transplanted with a 3D construct of CORR-R3381X MPCs in Promocell growth medium (6 months after transplantation). Human lamin A/C and human spectrin (both red) and human dystrophin (green). Nuclei were labeled with DAPI (blue). (B) Detection of human (a) and mouse (b) PAX7+ cells (arrowheads) in the satellite cell compartment at 5 months after transplantation. (a) A PAX7+ cell (green) of human origin labeled by human laminA/C (red) and DAPI (blue) adjacent to a human myofiber (hSpectrin, red) beneath the basal lamina (hLaminin β1, green). (b) A PAX7+ cell (green) of mouse origin with DAPI (blue) adjacent to a human myofiber (hSpectrin, red) within the basal lamina (hLaminin β1, green). (C) Innervation of donor-derived human myofibers (hDystrophin, red) in mdx nude mice at 5 months after transplantation. The formation of NMJs (arrowheads) is demonstrated by co-localization of post-synaptic marker AChR (labeled by α-Bungarotoxin, green) and pre-synaptic marker SV2 (cyan). Nuclei were labeled with DAPI (blue). (D) Vascularization in the engrafted regions at 6 months after transplantation as demonstrated by detection of blood vessels (CD31, red) adjacent to human myofibers (hDystrophin, green). Nuclei were labeled with DAPI (blue). 6 biological replicates for long-term engraftment experiment. Scale bars, 10–500 μm as indicated in each panel.

Article Snippet: CD31 (clone 390) , eBioscience , Cat#14-0311-81; RRID: AB_467200.

Techniques: Construct, Transplantation Assay, Labeling, Derivative Assay, Marker

Journal: Cell Reports Medicine

Article Title: Engineered human myogenic cells in hydrogels generate innervated vascularized myofibers within dystrophic mouse muscle on long-term engraftment

doi: 10.1016/j.xcrm.2025.102019

Figure Lengend Snippet:

Article Snippet: CD31 (clone 390) , eBioscience , Cat#14-0311-81; RRID: AB_467200.

Techniques: Plasmid Preparation, Recombinant, Modification, Saline, Fluorescence, Gene Expression, CRISPR, Derivative Assay, Software, Multiplex Assay

Journal: Cell Reports Medicine

Article Title: CDK12 loss drives prostate cancer progression, transcription-replication conflicts, and synthetic lethality with paralog CDK13

doi: 10.1016/j.xcrm.2024.101758

Figure Lengend Snippet:

Article Snippet: Rat monoclonal anti-mouse CD31 (390), PE-Cyanine7 , Thermo Fisher Scientific , Cat# 25-0311-82; RRID: AB_2716949.

Techniques: Virus, Recombinant, Plasmid Preparation, Blocking Assay, Lysis, Protease Inhibitor, Membrane, Transfection, SYBR Green Assay, Cell Viability Assay, Avidin-Biotin Assay, cDNA Synthesis, Software

Journal: Cell Reports Medicine

Article Title: CDK12 loss drives prostate cancer progression, transcription-replication conflicts, and synthetic lethality with paralog CDK13

doi: 10.1016/j.xcrm.2024.101758

Figure Lengend Snippet:

Article Snippet: Rat monoclonal anti-mouse CD31 (390), PE-Cyanine7 , Thermo Fisher Scientific , Cat# 25-0311-82; RRID: AB_2716949.

Techniques: Virus, Recombinant, Plasmid Preparation, Blocking Assay, Lysis, Protease Inhibitor, Membrane, Transfection, SYBR Green Assay, Cell Viability Assay, Avidin-Biotin Assay, Software